Ultrasensitive Protein Detection Powered by Single-molecule Precision
Ultrasensitive protein detection (USPD) is an advanced technology developed for Platinum Pro® that enables highly sensitive detection of low-abundance proteins. Optimized for targeted protein panels, USPD harnesses the extreme sensitivity of single-molecule detection to detect and quantify protein biomarkers with speed and sensitivity that exceed conventional methods.
Quantum-Si is exploring industry partnerships to advance USPD in neurological and inflammatory diseases and beyond.
SENSITIVITY
Detect low-abundance
neurological and inflammatory
biomarkers
SPEED
Sample to answer in ~2 hours
with one instrument
SIMPLICITY
Proteins detected directly in
serum, eliminating complex
sample prep
How It Works
USPD is suitable for detection of panels with up to ten proteins. It enables direct detection and analysis of individual protein molecules, using immobilized affinity reagents, labeled detection antibodies, and real-time signal evaluation.
Capture
Capture affinity reagents are attached to the wells of the chip, where proteins of interest are captured from biological samples.
Detect
Detection affinity reagents are added, resulting in the dynamic formation of single ternary complexes. Ultrasensitive detection of these events is achieved through repeated binding of an NAA recognizer.
Analyze
Each single-molecule sandwich complex is quantified in real time, enabling ultrasensitive determination of protein biomarker concentration.
Multiplex to Enable Proteoform Detection
The USPD workflow can be multiplexed using multiple recognizing antibodies detected with different Quantum-Si recognizers. We applied this approach to detect two different Tau isoforms, Tau 2N4R and 1N3R. In this experiment, anti-total Tau capture antibodies (HT7) were immobilized on the chip surface to capture both isoforms.
Two recognizing antibodies were then used to differentiate between tau isoforms 2N4R and 1N3R present in the same sample: Tau-12-R, derived from the Tau-12 antibody and tagged with an arginine peptide, and Anti-2N-F, derived from an Anti-2N antibody and tagged with a phenylalanine peptide. Both antibodies bind to the 2N4R isoform, while only Tau-12-R binds to the 1N3R isoform. This difference in antibody binding generates signal patterns that are distinct for each Tau isoform, enabling their discrimination using the Platinum Pro platform.
Interested in collaborating with Quantum-Si to advance USPD?
