Immunoprecipitation of IL-6 from Human Serum for Next-Generation Protein Sequencing™ on Platinum®


The enrichment of low-abundant proteins from complex biological matrices is a key step for many proteomic workflows. In this technical note, we present an immunoprecipitation protocol compatible with Quantum-Si’s next-generation protein sequencing™ workflow. This process utilizes an on-bead digestion protocol that forgoes the sample elution step and proceeds directly to the preparation of digested peptide libraries. We demonstrate the feasibility of this approach by specifically enriching IL-6 through immunoprecipitation from human serum, followed by protein sequencing and identification on Platinum®.