Protein Barcoding and Next-Generation Protein Sequencingu™

Explore our resources to discover how Platinum can elevate your antibody research and development:

• Immunoprecipitation of IL-6 from Human Serum for Next- Generation Protein Sequencing™ on Platinum® — APP NOTE

  The application note explores the enhancement of scarce proteins from complex biological matrices, a crucial phase in numerous proteomic workflows. It presents a cutting-edge immunoprecipitation method tailored for Quantum-Si’s advanced protein sequencing workflow. This on-bead digestion approach directly formulates digested peptide libraries, eliminating the need for a sample elution step. Its efficacy is demonstrated by enriching IL-6 from human serum and subsequently sequencing and identifying it with Platinum.rnrnSee the transformative power of this novel immunoprecipitation method by accessing the complete application note now.rnrnu003ca class=u0022wp-block-button__link has-black-color has-text-color wp-element-buttonu0022 href=u0022https://www.quantum-si.com/resources/app-notes/immunoprecipitation-of-il-6-from-human-serum-for-next-generation-protein-sequencing-on-platinum/u0022u003eDownload the Application Noteu003c/au003ernrnu003cimg class=u0022alignnone size-full wp-image-2427u0022 src=u0022https://www.quantum-si.com/wp-content/uploads/IL6-IP_AppNote_Antibody_Characterization.svgu0022 alt=u0022Antibody characterizationu0022 /u003ernrnThe application note showcases an all-inclusive process for on-bead digestion and immunoprecipitation of proteins, setting the stage for cutting-edge protein sequencing. This detailed procedure underscores the smooth progression from protein capture to its readiness for sophisticated sequencing, underscoring the method’s innovative and efficient nature.rnrnHere we highlight the nuanced data of traces, coverage, and pulse duration for peptides identified in IL-6 immunoprecipitated from PBS. Employing five recognizers, the research effectively pinpoints 12 amino acids, resulting in the detection of three unique peptides.rnu003cfigureu003eu003cimg class=u0022wp-image-1087u0022 src=u0022https://www.quantum-si.com/wp-content/uploads/2023/11/Immunoprecipitation-of-IL-6-from-Human-Serum-App-Note-Image-2.jpgu0022 sizes=u0022(max-width: 920px) 100vw, 920pxu0022 srcset=u0022https://www.quantum-si.com/wp-content/uploads/2023/11/Immunoprecipitation-of-IL-6-from-Human-Serum-App-Note-Image-2.jpg 920w, https://www.quantum-si.com/wp-content/uploads/2023/11/Immunoprecipitation-of-IL-6-from-Human-Serum-App-Note-Image-2-300×168.jpg 300w, https://www.quantum-si.com/wp-content/uploads/2023/11/Immunoprecipitation-of-IL-6-from-Human-Serum-App-Note-Image-2-768×429.jpg 768wu0022 alt=u0022u0022 width=u0022920u0022 height=u0022514u0022 /u003eu003c/figureu003ernu003cfigureu003eu003cimg class=u0022wp-image-1088u0022 src=u0022https://www.quantum-si.com/wp-content/uploads/2023/11/Immunoprecipitation-of-IL-6-from-Human-Serum-App-Note-Image-3-1024×532-1.jpgu0022 sizes=u0022(max-width: 1024px) 100vw, 1024pxu0022 srcset=u0022https://www.quantum-si.com/wp-content/uploads/2023/11/Immunoprecipitation-of-IL-6-from-Human-Serum-App-Note-Image-3-1024×532-1.jpg 1024w, https://www.quantum-si.com/wp-content/uploads/2023/11/Immunoprecipitation-of-IL-6-from-Human-Serum-App-Note-Image-3-1024×532-1-300×156.jpg 300w, https://www.quantum-si.com/wp-content/uploads/2023/11/Immunoprecipitation-of-IL-6-from-Human-Serum-App-Note-Image-3-1024×532-1-768×399.jpg 768wu0022 alt=u0022u0022 width=u00221024u0022 height=u0022532u0022 /u003eu003c/figureu003ernThis insightful analysis showcases the count of aligned IL-6 peptides found in each immunoprecipitated serum sample. Beyond highlighting the method’s efficacy, the data provides a deeper understanding of the correlation between input concentration and sequencing results.

• Identifying Monoclonal Antibodies with Quantum-Si’s Next-Generation Protein Sequencing™ Technology — APP NOTE

  Detecting rare monoclonal antibodies within a polyclonal antibody group is vital for grasping disease processes, diagnostics, among other aspects. While conventional techniques have their merits, they often entail significant expenses, intricacy, and lengthy protocols. This application note unveils Quantum-Si’s advanced protein sequencing approach using the Platinum device, offering a remedy to these hurdles and adeptly pinpointing these essential antibodies.rnrnDiscover this revolutionary approach and its prospective significance by accessing the complete application note.rnrnu003ca class=u0022wp-block-button__link has-black-color has-text-color wp-element-buttonu0022 href=u0022https://www.quantum-si.com/resources/app-notes/identifying-monoclonal-antibodies-with-quantum-sis-next-generation-protein-sequencing-technology/u0022u003eDownload the Application Noteu003c/au003ernu003cfigureu003eu003cimg class=u0022wp-image-1090u0022 src=u0022https://www.quantum-si.com/wp-content/uploads/2023/11/Identifying-Monoclonal-Antibodies-App-Note-Image-1-1536×1204-1-1024×803.jpgu0022 sizes=u0022(max-width: 1024px) 100vw, 1024pxu0022 srcset=u0022https://www.quantum-si.com/wp-content/uploads/2023/11/Identifying-Monoclonal-Antibodies-App-Note-Image-1-1536×1204-1-1024×803.jpg 1024w, https://www.quantum-si.com/wp-content/uploads/2023/11/Identifying-Monoclonal-Antibodies-App-Note-Image-1-1536×1204-1-300×235.jpg 300w, https://www.quantum-si.com/wp-content/uploads/2023/11/Identifying-Monoclonal-Antibodies-App-Note-Image-1-1536×1204-1-768×602.jpg 768w, https://www.quantum-si.com/wp-content/uploads/2023/11/Identifying-Monoclonal-Antibodies-App-Note-Image-1-1536×1204-1.jpg 1536wu0022 alt=u0022u0022 width=u00221024u0022 height=u0022803u0022 /u003eu003c/figureu003ernHere we highlight the sequencing data of distinct peptides originating from two separate monoclonal antibodies. Gathered over a span of 10 hours, the data illustrates the recognition segments (RS) with coverage denoted by the color intensity in each box, paired with the pulse duration distribution. This depiction aptly showcases the Platinum instrument’s proficiency in precise sequencing and pinpointing peptides exclusive to each antibody.

• Measuring Nanobody Kinetics at the Single-Molecule Level— POSTER

  Antibodies are a paradigm for high-affinity, protein-based binding reagents and are extremely important in biotechnological, diagnostic, and therapeutic applications. Of special interest are nanobodies, recombinant variable domains from heavy-chain-only antibodies.rnrnDespite their benefits, nanobodies have only recently been used in single-molecule assays, e.g., PAINT imaging, which require careful tuning of their kinetic properties. In this work, we demonstrate the detection of nanobody-antigen binding at the single-molecule level.rnrnrnu003ca class=u0022wp-block-button__link has-black-color has-text-color wp-element-buttonu0022 href=u0022/resources/posters/measuring-nanobody-kinetics-at-the-single-molecule-level/u0022u003eDownload Posteru003c/au003e

• Multiplexing Nanobody Kinetics Measurements at the Single-Molecule Level — POSTER

  Single-molecule methods provide useful insights into the intermolecular variations and functional differences of individual molecules and have revealed much about the complexity of biological processes. Today it is possible to monitor binding kinetics at the single molecule level in a highly parallel fashion using zero-mode waveguide (ZMW) arrays. This allows monitoring the binding/unbinding of fluorescently labelled molecules on millions of immobilized targets simultaneously, obtaining a full kinetic description of their interactions, offering a complementary picture to classical techniques but also uncovering important details that are missed in bulk studies.rnrnIn our work, we use ZMW arrays to study the binding kinetics of antibody-antigen interactions at the single-molecule level. Moreover, we demonstrate that it is possible to couple these binding kinetics measurements with single-molecule protein sequencing to multiplex kinetic analysis to panels of nanobodies. This new approach to the study of nanobodies will help us understand the sequence/function relationship in nanobodies and open new directions in nanobody affinity maturation.rnrnu003ca class=u0022wp-block-button__link has-black-color has-text-color wp-element-buttonu0022 href=u0022/resources/posters/multiplexing-nanobody-kinetics-measurements-at-the-single-molecule-level/u0022u003eDownload Posteru003c/au003e