Uncover New Insights with Post‑translational Modification (PTM) Analysis

See how Platinum Pro’s single-molecule resolution overcomes obstacles presented by other PTM analysis methods:

• Detecting Amino Acid Variants Using Next-Generation Protein Sequencing (NGPS) — PREPRINT PUBLICATION

  Next-Generation Protein Sequencing™ (NGPS) is a single-molecule approach for characterizing protein variants, offering detailed insight into proteoforms and amino acid substitutions not easily discerned by mass spectrometry. The novel data type produced by NGPS, which is based on binding of N-terminal amino acids by fluorescently tagged recognizer proteins, requires the development of new data analysis methods and bioinformatic tools.rnrnu003ca class=u0022wp-block-button__link has-black-color has-text-color wp-element-buttonu0022 href=u0022/resources/publications/detecting-amino-acid-variants-using-next-generation-protein-sequencing-ngps/u0022u003eDownload the Paperu003c/au003e

• Detection and Differentiation of PTMs with Platinum — APP NOTE

  u003cstrongu003eArginine PTM analysisu003c/strongu003ernrnArginine plays a critical role in protein structure and function and often mediates key interactions between protein binding partners or between proteins and DNA. The two most common arginine PTMs are citrullination and dimethylation, which respectively alter arginine into citrulline or ADMA and SDMA.rnu003cfigureu003eu003cimg class=u0022wp-image-306u0022 src=u0022/wp-content/uploads/2023/04/The-limitations-of-arginine-PTM-analysis-1024×524.webpu0022 sizes=u0022(max-width: 1024px) 100vw, 1024pxu0022 srcset=u0022/wp-content/uploads/2023/04/The-limitations-of-arginine-PTM-analysis-1024×524.webp 1024w, /wp-content/uploads/2023/04/The-limitations-of-arginine-PTM-analysis-300×154.webp 300w, /wp-content/uploads/2023/04/The-limitations-of-arginine-PTM-analysis-768×393.webp 768w, /wp-content/uploads/2023/04/The-limitations-of-arginine-PTM-analysis.webp 1190wu0022 alt=u0022u0022 width=u00221024u0022 height=u0022524u0022 /u003eu003c/figureu003ernADMA and SDMA isomers share structural similarities and an identical mass, limiting their identification through traditional mass spectrometry. Likewise, determining arginine to citrulline results in a negligible mass increase. Another common method for PTM detection is antibody-based immunoassay ELISA, but only a few antibodies against arginine PTMs are commercially available, restricting its application.rnrnu003cstrongu003eDirect detection of arginine PTMs in proteins with Platinumu003c/strongu003ernrnWith Platinum’s single-molecule resolution and sensitivity, you can detect and differentiate arginine PTMs accurately. Peptide fragments are probed in real-time by fluorescently labeled recognizers, designed to bind on and off to their cognate N-terminal amino acid (NAA), while aminopeptidases in solution remove analyzed NAAs to expose subsequent amino acids for further recognition.rnFluorescence lifetime, intensity, and kinetic data are collected in real-time and analyzed to determine amino acid sequence and PTM content. This novel single-molecule sequencing approach can overcome the challenges facing conventional methods through unparalleled amino acid detection.rnrnUsing Quantum-Si’s single-molecule protein sequencing, we demonstrated how to detect citrulline and how to differentiate between dimethylated arginine residues efficiently.rnrnu003ca class=u0022wp-block-button__link has-black-color has-text-color wp-element-buttonu0022 href=u0022/resources/app-notes/detecting-arginine-post-translational-modifications-using-quantum-si-next-generation-protein-sequencing-technology/u0022u003eSee the Datau003c/au003ernu003cfigureu003eu003cimg class=u0022wp-image-306u0022 src=u0022/wp-content/uploads/Detecting_Arginine_PTMs_Fig3.jpgu0022 sizes=u0022(max-width: 1024px) 100vw, 1024pxu0022 /u003ePlatinum Can Detect and Differentiate PTMs – u003ca href=u0022/resources/app-notes/detecting-arginine-post-translational-modifications-using-quantum-si-next-generation-protein-sequencing-technology/u0022u003eDownload the application noteu003c/au003e to see how the process works and the PTM analysis that Platinum® delivers.u003c/figureu003e

• Beyond the Genome: Identification of Protein Sequence and PTMs with Quantum-Si’s Next-Generation Protein Sequencer™ Platinum — POSTER

  To demonstrate the versatility of Platinum and the use of kinetic signatures, we sequenced various types of samples, including mixtures of recombinant proteins, peptides with post-translational modifications (PTMs), proteins immunoprecipitated from human serum, and proteins isolated from human serum via fractionation with SDS-PAGE. The resulting peptides generated distinct kinetic signatures aligned to their respective sequences, highlighting the efficacy of Quantum-Si’s sequencing platform in analyzing multi-protein mixtures at reduced input concentrations.rnrnu003ca class=u0022wp-block-button__link has-black-color has-text-color wp-element-buttonu0022 href=u0022/resources/posters/beyond-the-genome-identification-of-protein-sequence-and-ptms-with-quantum-sis-next-generation-protein-sequencer-platinum/u0022u003eDownload Posteru003c/au003e

• Beyond the Genome: Unraveling Protein Variability with Quantum-Si’s Next-Generation Protein Sequencing Technology — POSTER

  Protein sequencing is a groundbreaking advancement in proteomics that augments genomics and transcriptomics research by providing crucial insights into the functional proteins encoded by the genome. Protein sequencing offers a more complete understanding of cellular processes and disease mechanisms by detecting changes at the protein level, such as post-translational modifications (PTMs), which cannot be captured by genomics data alone. Next-Generation Protein Sequencing™ (NGPS) on Platinum® enables researchers to identify and characterize proteins with single-molecule resolution in a simple workflow and on a benchtop instrument.rnrnu003ca class=u0022wp-block-button__link has-black-color has-text-color wp-element-buttonu0022 href=u0022/resources/posters/beyond-the-genome-unraveling-protein-variability-with-quantum-sis-next-generation-protein-sequencing-technology-2/u0022u003eDownload Posteru003c/au003e