Protein Barcoding with Next-Gen Protein Sequencing™
Next-Generation Protein Sequencing™ (NGPS™) offers single-molecule sequencing of multiplexed protein barcodes with quantitative results.
Drug Delivery
Evaluate and optimize drug delivery vehicles like LNPs or AAVs with protein barcodes
Protein Engineering
Efficiently analyze protein characteristics in multiplex with protein barcodes
Advance Drug Delivery and Protein Analysis with Precision Barcoding
Characterize multiplexed drug delivery and translation efficiency of mRNA therapeutics with lipid nanoparticles (LNPs). Multiplexed analysis of target therapeutics and drug delivery systems accelerates
screening cycles, reduces resources of in vivo and in vitro models, and saves time.
Barcode mix encoded as mRNA and packaged into LNPs
Inject into mouse model; harvest target tissues
Enrich for target protein; functionalize, cleave, and sequence barcodes
How it Works
Protein barcoding provides users with a robust method to screen multiple protein variants in a single pooled reaction. Individual proteins are tagged with short, unique peptide barcodes that identify each variant. With the barcoding kit, screened proteins are prepared and barcodes are released for sequencing on Platinum®.
Prepare peptide barcodes for single-molecule sequencing
Sequence peptide barcodes with single-molecule precision
Analyze multiplexed barcodes with relative quantitation
Barcoding Kit
Provides methods for expression of unique peptide barcodes in proteins of interest for downstream sequencing and analysis on Platinum.
The Barcoding Kit contains reagents to prepare and immobilize barcode-tagged peptides for sequencing. Eight unique peptide barcodes are carefully designed for optimal sequencing performance with the Sequencing Kit.
Sequencing Kit
Contains ready-to-sequence chips, recognizers, and aminopeptidases.
When used with the Barcoding Kit, eight peptide barcodes can be uniquely identifed in a multiplexed sequencing run, providing relative quantitation with a tenfold dynamic range.
Explore our resources to discover how Next-Generation Protein Sequencing with Platinum can enhance your protein research:
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Next-Generation Protein Sequencing Kit — DATA SHEET
The Platinum instrument fits on your benchtop and seamlessly integrates into your existing workflow, enabling protein sequencing right after sample preparation and chip loading.
Equipped with a user-friendly interface, Platinum allows you to easily start your protein sequencing run following a few prompts. Sequencing data is generated and can be automatically transferred to the Platinum Analysis Software for analysis.
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Barcoding Applications — WEBPAGE
This webpage explains how protein barcoding — combined with Quantum-Si’s Next-Generation Protein Sequencing (NGPS) solution — offers a transformative solution by enabling multiplexed protein variant screening and characterization with significantly faster assay speeds and enhanced throughput.
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Overcoming the RNA Therapeutics Delivery Challenge — CASE STUDY
Explore this case study to learn how researchers are using the protein barcoding application on Platinum® to accelerate development of novel gene therapies with improved targeting capabilities. With protein barcoding you can:
- Accelerate the screening and optimization of lipid nanoparticle (LNP) delivery vehicles for targeted gene therapies.
- Precisely track LNP delivery to specific tissues, enhancing precision and efficiency.
- Utilize a cost-effective method as an accessible alternative to mass spectrometry for in vivo delivery studies.
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Use of Protein Barcodes & Next Generation Protein Sequencing™
for High-Throughput Mapping of Protein Conformations & Their Phenotypic Effects — CASE STUDY
Explore the case study to understand how combining next-generation protein sequencing with protein barcoding facilitates high-throughput mapping of protein conformations and phenotypic effects, without the limitations of traditional methods. With protein barcoding, you can:
- Conveniently screen hundreds of variants using protein barcoding in your protein expression system
- Utilize the diversity of protein barcodes to screen proteins with diverse characteristics
- Expand your research and uncover innovative strategies for preventing and treating various diseases
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Protein Barcodes for Next-Generation Protein Sequencing™ — APP NOTE
Discover the power of protein barcoding with Quantum-Si’s Next-Generation Protein Sequencing™ on the Platinum® instrument. This innovative technology combines the accessibility of DNA-based methods with the versatility of protein barcoding. By attaching peptides to macromolecular linkers and employing dye-labeled recognizers and aminopeptidases, researchers can generate distinct kinetic signatures for each peptide, enabling high-confidence sequence alignment. Explore our application note for criteria on peptide barcode design, library generation methods, and practical examples of how peptide barcodes revolutionize protein screening, including a 300-fold enrichment in nanobody abundance after positive selection using protein barcodes.