Discover Protein Variants with Single-molecule Resolution on Platinum™

Dive into our application notes to grasp how Platinum’s next-generation sequencing optimizes protein variant detection with unparalleled precision.

• Quantum-Si’s Next Generation Protein Sequencing Technology Enables Rapid and Accurate Distinction of Variants of the SARS-CoV-2 Virus – APP NOTE

  This application note showcases Quantum-Si’s protein sequencing technology on the Platinum instrument, which enables the rapid and accurate distinction of variants of the SARS-CoV-2 virus based on differences in the amino acid sequence of their Spike proteins. By identifying and tracking these variants, scientists can better understand their impact on public health and develop effective treatments and vaccines.

  Download the application note to learn more about this groundbreaking technology.

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  Focusing on a key mutation, we observed the distinctive kinetic influences on recognizer binding. The clear differences in pulse durations (PD) between the peptides of interest highlight the power of Quantum-Si’s Platinum instrument in differentiating the Alpha variant from the Delta and Omicron variants through a single mutation.

  Here we highlight the differentiation of the Omicron variant from the Alpha and Delta variants based on the detection of a single peptide. The capability to identify variant-specific peptides contributes to the accurate and precise distinction of SARS-CoV-2 variants using Quantum-Si’s Platinum instrument.

  

• Single-Molecule Protein Sequencing on the Quantum-Si Platform: Advances in Protein and Proteoform Identification and Comparison With Mass Spectrometry – POSTERS

  Studies of the proteome would benefit greatly from methods to directly sequence and digitally quantify proteins and detect post-translational modifications with single-molecule sensitivity. Quantum-Si™ has developed the Platinum™ next-generation protein sequencing™ platform to offer a convenient benchtop solution for direct sequencing of single protein molecules with the aim of making protein sequencing accessible to any research lab. Quantum-Si has improved fundamentals of the assay components and platform allowing for increased coverage across the proteome.

  • This poster describes these Platinum improvements and how they have resulted in unprecedented read depth and the identification of previously unseen proteins, leading to new insights and greater confidence in protein identification.

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• Platinum® Next-Generation Protein Sequencing Advanced Data Analysis – TECHNICAL NOTE

  Next-Generation Protein Sequencing™ (NGPS) on Platinum® enables protein identification and characterization with single-molecule and single amino acid resolution in a simple workflow using a benchtop instrument. Platinum Analysis Software workflows make peptide alignment and protein mapping easy to interpret without the need for advanced expertise. The output files from Platinum Analysis Software provide researchers with the opportunity to perform advanced analysis on NGPS data including analysis of amino acid variants and modifications based on kinetic signatures.

  • This technical note provides guidance on interpreting results from Primary Analysis and Peptide Alignment Workflows and performing advanced analysis on NGPS data generated by Platinum.

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• Next-Generation Protein Sequencing™: Interrogating Proteins with Single-molecule Amino Acid Resolution on Platinum® – WEBINARS

  Proteins are the workhorses of cells and provide vital insights to cell health and disease function. Immunoassays and mass spectrometry are well established methods for studying proteins and have led to important discoveries in human health and disease. However, these techniques either lack single-molecule resolution to interrogate each individual amino acid residue or require a large investment in equipment and lab space, driving the need for a simple benchtop solution with single-molecule resolution for protein identification and interrogation.

  In this webinar, we will explore how next-generation protein sequencing on Platinum works and how it can be used to interrogate proteins with single-molecular amino acid resolution. This new technology utilizes semiconductor chip technology and fluorescently labeled n-terminal amino acid (NAA) recognizers to identify individual amino acids in a protein. The binding kinetics and order of the NAA recognizer signal results in a unique kinetic signature for each amino acid. Aminopeptidases then cleave the n-terminus and expose the next amino acid for binding. This process repeats until each peptide is sequenced.

  We will demonstrate how this technology can be used with traditional protein workflows that isolate proteins of interest and interrogate their sequences with single-molecule resolution. Finally, we will discuss the scalability of this technology for advancing our understanding of proteins and offering insights to human health and disease.

  Learning Objectives:

  • Explore how next-generation protein sequencing works to interrogate amino acid residues
  • Understand how next-generation protein sequencing can be used with other lab methods to understand protein biology
  • Learn how next-generation protein sequencing can be used to detect amino acid variants with single-molecule resolution

  Speakers: Ben Moree, PhD, QSI

  Date: December 7, 2023

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• Next-Generation Protein Sequencing™ Advances Variant Identification – WHITEPAPERS

  In their quest to fully understand the proteome, researchers face an identification challenge: Distinguishing individual proteins among the 20,000-plus proteins and over 1 million proteoforms in human cells. Now a benchtop sequencer provides easy access to the analytical resolution needed to continue mapping protein variation.

  Platinum®, the Next-Generation Protein Sequencing™ platform, provides unambiguous amino acid detection at single molecule resolution. Learn how it complements existing protein identification techniques, such as mass spectrometry and immunoassays, while providing deeper insights with unprecedented accessibility.

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